Culdocentesis
Posted October 1, 2022 by Anusha ‐ 2 min read
Culdocentesis is a diagnostic procedure to remove abnormal fluid from behind your vagina in your cul-de-sac or pouch of Douglas. It’s not as widely used as it once was, but can help diagnose infection or medical conditions.
What is Culdocentesis?
- Culdocentesis is a medical procedure that checks for abnormal fluid behind your vagina and under your uterus in an area called the posterior cul-de-sac. 
- Your healthcare provider inserts a thin needle through your vaginal wall to obtain a sample of fluid from this area for testing. 
- Transvaginal ultrasound has largely replaced culdocentesis because it can detect abdominal and pelvic fluid. 
- Surgeons may use minimally invasive or image-guided surgical techniques instead of culdocentesis. 
When is Culdocentesis performed?
- Certain medical conditions and infections cause irregular fluid to fill your posterior cul-de-sac. 
- Most healthcare providers use an ultrasound to detect fluid behind your uterus. 
- If they suspect the fluid is abnormal, they may perform culdocentesis to extract fluid for testing. 
Some reasons for culdocentesis are:
- Rupturing of an ectopic pregnancy. 
- Pelvic inflammatory disease (PID). 
- Ruptured ovarian cysts. 
- Endometriosis. 
- Uterine fibroids. 
- Trauma to the pelvis. 
- Pain in your pelvis or lower abdomen. 
Procedure of Culdocentesis
The steps of the procedure typically involve:
- Your healthcare provider will perform a pelvic exam and insert a surgical instrument to lift your cervix up. This shouldn’t be painful but may be uncomfortable. 
- You provider numbs an area of your cervix with a local anesthesia. 
- Your provider inserts a thin needle through the wall of your vagina below where your cervix ends. The needle is attached to a syringe that collects fluid. 
- Finally, your provider removes a sample of fluid from your posterior cul-de-sac through the needle. 
What do the results of Culdocentesis mean?
- A normal result means your fluid is clear. 
- Bloody, pus-filled or large amounts of clear fluid may suggest there’s a problem. 
- For example, yellow, pus-filled fluid indicates an infection. 
- Blood that doesn’t clot means an ectopic pregnancy has ruptured. 
- This is because blood from your cul-de-sac area doesn’t clot. 
- The fluid may need drained or treated with antibiotics depending on the test results. 
How is ectopic pregnancy diagnosed using Culdocentesis?
- This procedure was once valuable in diagnosing ruptured ectopic pregnancies with hemoperitoneum (bleeding) when ultrasound wasn’t yet widely available. 
- During this time, ectopic pregnancies weren’t detected until they ruptured. 
- However, ultrasound can detect an ectopic pregnancy before it ruptures which reduces the need for culdocentesis. 
Risks of Culdocentesis
Risks of culdocentesis include:
- Puncturing your uterus or rectum. 
- Infection 
- Puncturing blood vessels, cysts or tumors.